Real-world disease and parasite monitoring is often hampered by the inability of traditional approaches to easily sample broad geographical areas and large numbers of individuals. This can result in patchy data that fall short of what researchers need to anticipate and address outbreaks. Writing in BioScience, Jessica Farrell (University of Florida), Liam Whitmore (University of Limerick), and David Duffy (University of Florida) describe the promise of novel molecular techniques to overcome these shortcomings.