The doctoral thesis of A. B. M. Kaiser Manjur, M.Sc., at the Faculty of Health Sciences, provides novel insights into two medically important transcription factors (TFs), glucocorticoid receptor (GR) and androgen receptor (AR)—in particular, their functional interactions with two coregulator proteins, IRF2BP2 (Interferon regulatory factor 2 binding protein 2) and BCOR (B-cell lymphoma 6 corepressor). State-of-the-art genome-wide methods were utilized to define the role of these coregulators in modulating GR and AR target gene expression. Importantly, the findings of this thesis revealed target gene-dependent role of coregulators in GR and AR signaling and provided insights for designing and developing new drugs to treat inflammatory diseases and prostate cancer.